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07/10/2014
Activity of antibacterial compounds immobilised on montmorillonite

ACTIVITY OF ANTIBACTERIAL COMPOUNDS IMMOBILISED ON MONTMORILLONITE
Abstract
The activities of antibacterial compounds, such as cetylpyridinium (CP), cetyltrimethylammonium (CTA), silver ions and metallic silver, immobilised on montmorillonite (MMT), were tested in Escherichia coli and Enterococcus faecium bacteria. The results of bacterial growth tests were confirmed by determination of the minimum inhibition concentrations (MICs). Unlike CP and CTA, the intercalated silver ions were easily released from MMT by ion-exchange with Na+ and acted as very effective antibacterial substances in the long term. Their bactericidal and bacteriostatic effects were determined. Generally, antibacterial compounds are effective when they are released from an inorganic carrier. Metallic silver was prepared by reduction of intercalated Ag+ with sodium borohydride. Antibacterial effects of metallic silver were not observed.

Keywords
Antibacterial activity; Montmorillonite; Cationic surfactants; Silver; Bacteria Soundsystem Crew

The activities of antibacterial compounds, such as cetylpyridinium (CP), cetyltrimethylammonium (CTA), silver ions and metallic silver, immobilised on montmorillonite (MMT), were tested in Escherichia coli and Enterococcus faecium bacteria

Simple new synthesis of copper nanoparticles in water/acetonitrile mixed solvent and their characterizationMd. Abdulla-A...
07/10/2014
Simple new synthesis of copper nanoparticles in water/acetonitrile mixed solvent and their...

Simple new synthesis of copper nanoparticles in water/acetonitrile mixed solvent and their characterization
Md. Abdulla-Al-Mamun, Yoshihumi Kusumoto*, Manickavachagam Muruganandham
Department of Chemistry and Bioscience, Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065,Japan

Abstract
Copper nanoparticles were successfully synthesized by the borohydride reduction of copper nitrate salt in water/CH3CN mixed solvent under inert argon-purged conditions. Cu nanoparticles were synthesized in large-scale production for the first time by introducing CH3CN into water and preventing oxidation during the preparation of nanoparticles. Nanoparticles were characterized by using UV–visible absorption spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) techniques. High resolution TEM pictures showed the formation of homogeneous cubic-structured copper nanoparticles with sizes less than 100 nm. This new kind of synthesis method shows the excellent stability compared with that of citrate-protected copper nanoparticles, which may provide an efficient way to improve the fine tuning of the structure and size of copper nanoparticles.

Copper nanoparticles were successfully synthesized by the borohydride reduction of copper nitrate salt in water/CH3CN mixed solvent under inert argon-purged conditions

Cytotoxicity evaluation and antimicrobial studies of starch capped water soluble copper nanoparticlesAbstractWater solub...
07/09/2014
Cytotoxicity evaluation and antimicrobial studies of starch capped water soluble copper...

Cytotoxicity evaluation and antimicrobial studies of starch capped water soluble copper nanoparticles
Abstract
Water soluble monodisperse copper nanoparticles of about 10 nm diameter were prepared by microwave irradiation using starch as green capping agent. The resulting Cu–starch conjugate were characterized by FTIR and energy dispersive X-ray analysis (EDX). The study confirmed the presence of copper embedded in polysaccharide matrix. The aqueous solution of starch capped copper nanoparticles (SCuNPs) exhibited excellent bactericidal action against both gram negative and gram positive bacteria. The in vitro cytotoxicity evaluation of the nanoparticles was carried out using mouse embryonic fibroblast (3T3L1) cells by MTT cell viability assay, extracellular lactate dehydrogenase (LDH) release and dark field microscopy imaging. The capped nanoparticles exhibited cytotoxicity at much higher concentration compared to cupric ions. Minimum bactericidal concentration (MBC) of SCuNPs was well below the in vitro cytotoxic concentration. Statistical analysis demonstrated p < 0.05 for significant results and p > 0.05 for non-significant ones as compared to untreated cells. The non-cytotoxic green Cu–starch conjugate offers a rational approach towards antimicrobial application and for integration to biomedical devices.

Water soluble monodisperse copper nanoparticles of about 10 nm diameter were prepared by microwave irradiation using starch as green capping agent. The resulting Cu–starch conjugate were characterized by FTIR and energy dispersive X-ray analysis (EDX)

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